Kinases (EC 2.7.x.x): Introduction

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Kinases belong to a large and diverse superfamily of enzymes [10,17] and members of the eukaryotic protein kinase (ePK) family are responsible for mediating and controlling the majority of complex signalling events in eukaryotic cells.

Reversible phosphorylation, where kinases transfer the gamma phosphate of ATP to hydroxyl groups of various substrates including lipids, sugars or amino acids plays an essential role in most, if not all, cellular signaling [29-30], influencing almost every cellular process (including regulation of the cell cycle, growth, apoptosis and signal transduction) [2,4,29]. In eukaryotes, reversible protein phosphorylation is often transient and is performed by ePKs on Ser, Thr or Tyr residues and the respective phosphatases, which remove the phosphate.

At the latest count, the human kinome contains 566 ePK genes. There are two main classes of human protein kinases: the protein tyrosine kinases (PTKs) which phosphorylate Tyr and the Ser- and Thr-specific kinases (STKs) which phosphorylate Ser and/or Thr residues on protein substrates [4-5,29]. Whilst there are a few dual-specificity protein kinases (phosphorylating both Tyr and Thr), the majority of ePKs are STKs. This is reflected in the ratio of cellular phosphorylation of pSer : pThr : pTyr = 1000 : 100 : 1 [2,4-5,14,16,22]. Although only a minor number of substrates are phosphorylated by PTKs, the importance of tyrosine phosphorylation is profound [2,5,11,14,16,22]. Many gain of function (GOF) and/or loss of function (LOF) mutations are found in PTKs which have, therefore, long been considered as important drug targets [2,4-5,11-12,16,19].

The ePK domain is the most abundant catalytic domain in the eukaryotic genome and the overall structural organization of the kinase domain is highly conserved [22]. The catalytic function of ePKs is confined to a ~300 amino acid domain, which provides all of the machinery required for phosphorylation of proteins on Ser, Thr and/or Tyr residues [11,17-18,22,24,26-27,31]. Of special note is the so-called "gatekeeper" residue which controls access to the "back-pocket" of the kinase ATP binding site, [8,18,24,26-27]since this residue is often mutated in kinase alleles resistant to inhibitors.

Aberrant kinase function, such as hyperactivity or overexpression plays a role in a wide variety of diseases including cancer, inflammatory diseases, diabetes, atherosclerosis, and immunological disorders [2-4,11,25]. Despite a third of all protein targets under investigation in the pharmaceutical industry being protein or lipid kinases, their full potential remains to be fully exploited [12].


Potent but non-selective tool molecules such as staurosporine led to the initiation of drug development efforts which culminated in the approval of the first kinase inhibitor, Imatinib (CGP57148, STI571, Glivec, Gleevec), for the treatment of chronic myelogenous leukemia (CML) in 2001. Since then, 42 small molecule kinase inhibitors have been approved by the FDA, the majority being for the treatment of cancer with a minor number approved for other indications. The figure below charts the last 32 years of kinase inhibitor drug discovery, and provides a timeline of kinase inhibitor drug approvals.

Although the structural determinants of kinase inhibition by small molecules binding to the ATP-binding site is well understood [7,9,11,20,28,31], the selectivity and the limited set of chemical entities targeting the ATP-binding site, have become the major issues in kinase drug discovery.

Traditionally, drug discovery has used in vitro biochemical- and cellular assays followed by in vivo clinical studies to assess drug efficacy. There are many different ways to measure biochemical protein kinase activity (such as detection of radio labeled transfer of phosphate to the substrate, ATP consumption or ADP production measurement, TR-FRET, peptide array-based, micro fluidic technologies, and label free analysis). Cellular assays show similar diversity, focused on measuring target modification (by Western blot, phospho-ELISA and reversed-phase arrays, for example) or changes in downstream signaling. Combining these various techniques gives a readout of in vitro kinase activity and the effect of inhibitors, and can be used to reveal on- and off-target effects. Such systematic inhibitor profiling has provided novel ways to better define the selectivity profile of drug candidates and has revealed previously unidentified mechanisms of action [1,23]. But, despite this enhanced profiling of drug candidates, it still proves very difficult to align these results with their potential toxological effects and clinical efficacy in patients, meaning that many potential research leads fail in pre-clinical stages of development. Recent progress in molecular profiling in conjunction with precision medicine will further our understanding and allow better assessment and prediction of efficacy/toxicity of these inhibitors in disease models (pharmacokinetics/pharmacodynamics) and patients [6,11,13,21,31]. The prospect of using kinase inhibitors against diseases other than cancer may also be enhanced by such improved understanding.

Agents other than small molecule compounds are being developed to target kinases.

Monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs)

The table below lists currently approved receptor tyrosine kinase mAbs, and indicates target specificity and clinical indications for which the mAbs can be used.

mAbsTrade nameTargetIndication
Ramucirumab (Cyramza)CyramzaVEGFR-2Advanced/metastatic gastric or gastroesophageal junction adenocarcinoma; metastatic non-small cell lung cancer; metastatic colorectal cancer
Panitumumab (Vectibix)VectibixEGFREGFR-expressing colorectal cancer
Pertuzumab (Perjeta)PerjetaHER2Metastatic HER2-overexpressing breast cancer
Cetuximab (Erbitux)ErbituxEGFRMetastatic KRAS negative colorectal cancer; squamous cell carcinoma of the head and neck
Trastuzumab Herceptin, HerclonHER2HER2/neu overexpressing breast cancer, some gastric adenocarcinomas
Ado-trastuzumab emtansineKadcylaHER2Metastatic HER2 over-expressing breast cancer

Kinase degraders

Proteolysis targeting chimeras (a.k.a. PROTACs) are dual domain molecules that direct targeted proteins towards the intracellular proteolytic degradation pathway. So in essence these agents reduce the physical amount of target protein present, rather than inhibiting its activity. PROTAC degraders work by recruiting an E3 ligase to the unwanted target protein, and this is then recognised and removed by the cells' ubiquitin/proteasome machinery. Degraders can be used against any disease-causing proteins. One kinase specific example is the CDK8 degrader JH-XI-10-02 (which in this case is also a CDK8 inhibitor) [15]. JH-XI-10-02 is a CDK8 inhibitor/pomalidomide chimeric molecule that binds to the ubiquitously expressed E3 ligase receptor cereblon via pomalidomide. JH-XI-10-02 thereby recruits E3 ligase CRL4cereblon to inhibitor-bound CDK8 and directs its destruction via cereblon-mediated ubiquitination and proteosomal degradation.


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Kinases (EC 2.7.x.x), introduction. Last modified on 18/04/2018. Accessed on 21/05/2018. IUPHAR/BPS Guide to PHARMACOLOGY,